| Registro completo |
| Provedor de dados: |
Biological Sciences
|
| País: |
Brazil
|
| Título: |
Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae) - doi: 10.4025/actascibiolsci.v34i1.6961
|
| Autores: |
Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENÓRBIO
Vanderlei, Edfranck de Sousa Oliveira; Universidade Federal do Ceará - UFC
Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará - UFC
Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará - UFC
Bessa, Érika Façanha; Universidade Federal do Ceará - UFC
Benevides, Norma Maria Barros; Universidade Federal do Ceará - UFC
|
| Data: |
2011-11-03
|
| Ano: |
2011
|
| Palavras-chave: |
2.08.01.00-9 Halymeniales
Sulfated macromolecules
Drying methods
Blood coagulation 2.08.01.00-9
|
| Resumo: |
Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal human plasma and compared to heparin (HEP) (193.00 IU mg-1). There was a difference in TSP yield of 19.05% and similar chromatographic SP profiles. Electrophoresis revealed fractions with distinct resolutions. The fractions eluted with 0.75 M of salt (M I and II) were the most active, measuring 27.40 and 72.66 IU mg-1, respectively, when compared to HEP. Therefore, obtaining SP with anticoagulant activity from Halymenia sp. is more efficient by freeze-drying.
|
| Idioma: |
Português
|
| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961
10.4025/actascibiolsci.v34i1.6961
|
| Editor: |
Universidade Estadual De Maringá
|
| Formato: |
application/pdf
application/pdf
|
| Fonte: |
Acta Scientiarum. Biological Sciences; v. 34, n. 1 (2012); 5-11
Acta Scientiarum. Biological Sciences; v. 34, n. 1 (2012); 5-11
|
| Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
|
|
